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抗生素微生物检定法——培养基及其制备方法
2008-10-20 13:24:21 青岛海博 
   
   培养基I                                                                
    胨            5g       琼脂          15~20g                            
    牛肉浸出粉    3g       水            1000ml                             
    磷酸氢二钾    3g                                                        
    除琼脂外,混合上述成分,调节pH使比最终的pH值略高0.2~0.4,加入琼脂,加热
溶化后滤过,调节pH值使灭菌后为7.8~8.0或6.5~6.6,在115℃灭菌30分钟。 
    培养基Ⅱ                                                                
    胨            6g       葡萄糖        1g                                
    牛肉浸出粉    1.5g     琼脂          15~20g                           
    酵母浸出粉    6g       水            1000ml                            
    除琼脂和葡萄糖外,混合上述成分,调节pH使比最终的pH值略高0.2~0.4,加入琼
脂,加热溶化后滤过,加葡萄糖溶解后,摇匀,调节pH值使灭菌后为7.8~8.0或6.5~6.6,
在115℃灭菌30分钟。    
    培养基Ⅲ                                                             
    胨            5g       磷酸氢二钾    3.68g                            
    牛肉浸出粉    1.5g     磷酸二氢钾    1.32g                            
    酵母浸出粉    3g       葡萄糖        1g                               
    氯化钠        3.5g     水            1000ml          
    除葡萄糖外,混合上述成分,加热溶化后滤过,加葡萄糖溶解后,摇匀,调节pH值
使灭菌后为7.0~7.2,在115℃灭菌30分钟。          
    培养基Ⅳ                                                        
    胨            10g      葡萄糖        10g                       
    氯化钠        10g      琼脂          20~30g                 
    枸橼酸钠      10g      水            1000ml                 
    除琼脂和葡萄糖外,混合上述成分,调节pH使比最终的pH值略高0.2~0.4,加入琼
脂,在109℃加热15分钟,于70℃以上保温静置1小时后滤过,加葡萄糖溶解后,摇匀,
调节pH值使灭菌后为6.0~6.2,在115℃灭菌30分钟。                  
    培养基Ⅴ                                                        
    胨            10g      琼脂          15~20g                      
    麦芽糖        40g      水            1000ml                  
    除琼脂和麦芽糖外,混合上述成分,调节pH使比最终的pH值略高 0.2~0.4,加入琼
脂,加热溶化后滤过,加麦芽糖溶解后,摇匀, 调节pH值使灭菌后为7.2~7.4,按需要
分装,在115℃灭菌30分钟,趁热斜放使凝固成斜面。                  
  培养基Ⅵ                                                        
  胨       8g    磷酸二氢钾  1g                 
  牛肉浸出粉   3g    葡萄糖    2.5g                 
  酵母浸出粉   5g    琼脂       15~20g               
  氯化钠     45g    水       1000ml            
  磷酸氢二钾   3.3g                                            
  除琼脂和葡萄糖外,混合上述成分,调节pH使比最终的pH值略高0.2~0.4,加入琼
脂,加热溶化后滤过,加葡萄糖溶解后,摇匀,调节pH值使灭菌后为7.2~7.4,在115℃
灭菌30分钟。
    培养基Ⅶ                                                        
    胨            5g        枸橼酸钠     10g            
    牛肉浸出粉    3g        琼脂         15~20g          
    磷酸氢二钾    7g        水      1000ml      
    磷酸二氢钾    3g                                              
    除琼脂外,混合上述成分,调节pH使比最终的pH值略高0.2~0.4,加入琼脂,加热
溶化后滤过,调节pH值使灭菌后为6.5~6.6,在115℃灭菌30分钟。            
    培养基Ⅷ                                                        
    酵母浸出粉    1g        琼脂                 15~20g        
    硫酸铵        1g        磷酸盐缓冲液(pH6.0)  1000ml  
    葡萄糖        5g                                            
  混合上述成分,加热溶化后滤过,在115℃灭菌30分钟。            
    营养琼脂培养基                                                  
    胨            10g       琼脂         15~20g                     
    氯化钠        5g        肉浸液       1000ml                 
    除琼脂外,混合上述成分,调节pH使比最终的pH值略高0.2~0.4,加入琼脂,加热
溶化后滤过,调节pH值使灭菌后为 7.2~7.4,分装,在115℃灭菌30分钟,趁热斜放使
凝固成斜面。
    培养基可以采用相同成分的干燥培养基代替,临用时,照使用说明配制和灭菌,备
用。
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