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出口食品中弯曲杆菌检验方法
2009-2-17 13:10:34 青岛海博生物
一、培养基明细:
   名称                            规格                   价格(元)
弯曲杆菌增菌肉汤                     *                       *
布氏肉汤                            250g                     90
布氏琼脂                            250g                     95
改良Skirrow氏培养基                 250g                     95
含0.18%琼脂的半固体布氏肉汤         250g                     100
营养肉汤                            250g                     70
中性红溶液                            *                       *
3%过氧化氢溶液                        *                       *
氧化酶试验试剂                        *                       *
亚硝酸盐检测(硝酸盐还原)试剂        *                       * 
乙酸铅纸条                            *                       *  
硝酸钾                                *                       *
甘氨酸                                *                       *
氯化钠                               500g                    6
盐酸半胱氨酸                          *                       *
马尿酸钠                              *                       *
萘啶酮酸                              *                       25
头孢菌新素                            *                        *
茚三酮                                *                        *
丙酮                                 500ml                     12
丁醇                                  *                        *
二、备注:
1、依据SN 0175—92出口食品中弯曲杆菌检验方法 适用于出口畜肉、禽肉、水产品和牛奶中弯曲杆菌检验。其它出口食品可参照使用。
2、弯曲杆菌增菌肉汤:
配方:
基础液:布氏肉汤
FBP浓原液:硫酸亚铁    2.5g
           焦亚硫酸钠  2.5g
           丙酮酸钠    2.5g
  将各成分溶于100ml蒸馏水中,经0.20um滤膜过滤除菌,存于4℃,每100ml经灭菌的基础液中加1ml,混匀。
1号抗生素溶液:万古霉素                        0.75g
               三甲氧苄氨嘧啶乳酸盐            0.38g
               多黏菌素B                      250000IU
  将各成分溶于500ml蒸馏水中,经0.20um滤膜过滤除菌,每100ml经灭菌的基础液中加1ml。
2号抗生素溶液:利福平                0.2g
               三甲氧苄氨嘧啶乳酸盐  0.2g
               多黏菌素B            200000IU
               放线菌酮              2.0g
  将各成分置200ml容量瓶置,加入95%乙醇50ml,振摇使溶解,加入蒸馏水至200ml。过滤除菌,每100ml经灭菌的基础液中加1ml。
3、中性红溶液:将中性红2.0g溶于1000ml蒸馏水中,在培养基灭菌前加入,每100ml培养基中加入1ml。
4、亚硝酸盐检测(硝酸盐还原)试剂 
   甲液:将0.02gN-(1萘)乙二胺盐酸盐加于100ml 1.5mol/l盐酸中,在通风柜中微加热溶解。
   乙液:将1.0g对氨基苯磺酸加于100ml 1.5mol/l盐酸中,在通风柜中微加热溶解。   
   
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